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Št. zadetkov: 7
Development of adaptive resistance to electric pulsed field treatment in CHO cell line in vitro
Tamara Polajžer, Damijan Miklavčič
Izvirni znanstveni članek
Oznake: elektroporacija;zdravljenje z električnimi pulzi;ponovno zdravljenje;rezistenca;electroporation;pulsed electric field treatment;repeated treatments;resistance;
Pulsed electric field treatment has increased over the last few decades with successful translation from in vitro studies into different medical treatments like electrochemotherapy, irreversible electroporation for tumor and cardiac tissue ablation and gene electrotransfer for gene therapy and DNA v ...
Leto: 2020 Vir: Fakulteta za elektrotehniko (UL FE)
Primerjava učinkov visokofrekvenčnih bipolarnih in nizkofrekvenčnih monopolarnih električnih pulzov na preživetje celice
Tamara Polajžer, Damijan Miklavčič
Doktorska disertacija
Oznake: elektroporacija;ireverzibilna elektroporacija;povečana prepustnost membrane;visokofrekvenčni bipolarni pulzi;DAMP molekule;imunogena celična smrt;doktorske disertacije;
Elektroporacija je pojav, kjer izpostavljenost celice zunanjemu električnemu polju povzroči povečano prepustnost membrane, zato v celico lahko vstopijo molekule, za katere je drugače membrana neprepustna. Če po prenehanju delovanja električnega polja celica preživi govorimo o reverzibilni elektropor ...
Leto: 2022 Vir: Fakulteta za elektrotehniko (UL FE)
Cell death due to electroporation
Tina Batista Napotnik, Tamara Polajžer, Damijan Miklavčič
Pregledni znanstveni članek
Oznake: apoptoza;celična smrt;poškodba celice;elektroporacija;celjenje membrane;nekroptoza;nekroza;piroptoza;apoptosis;cell death;cell injury;electroporation;membrane repair;necroptosis;necrosis;pyroptosis;
Exposure of cells to high voltage electric pulses increases transiently membrane permeability through membrane electroporation. Electroporation can be reversible and is used in gene transfer and enhanced drug delivery but can also lead to cell death. Electroporation resulting in cell death (termed a ...
Leto: 2021 Vir: Fakulteta za elektrotehniko (UL FE)
Dynamics of cell death due to electroporation using diferent pulse parameters as revealed by diferent viability assays
Wencheng Peng, Tamara Polajžer, Chenguo Yao, Damijan Miklavčič
Izvirni znanstveni članek
Oznake: elektroporacija;ablacija s pulznim električnim poljem;dinamika celične smrti;parametri pulza;testi preživetja;electroporation;pulsed field ablation;cell death dynamics;pulse parameters;viability assays;
The mechanisms of cell death due to electroporation are still not well understood. Recent studies suggest that cell death due to electroporation is not an immediate all-or-nothing response but rather a dynamic process that occurs over a prolonged period of time. To investigate whether the dynamics o ...
Leto: 2024 Vir: Fakulteta za elektrotehniko (UL FE)
Efficient gene transfection by electroporation - in vitro and in silico study of pulse parameters
Tjaša Potočnik, Shaurya Sachdev, Tamara Polajžer, Alenka Maček Lebar, Damijan Miklavčič
Izvirni znanstveni članek
Oznake: genski elektroprenos;nanosekundni pulzi;visokofrekvenčni bipolarni pulzi;elektroporacija;plazmidna DNA;gene electrotransfer;nanosecond pulses;high frequency bipolar pulses;electroporation;plasmid DNA;
Gene electrotransfer (GET) is a widely used method for nucleic acids’ delivery into cells. We explored, evaluated, and demonstrated the potential use of different pulse durations for introducing plasmid DNA (pDNA) into cells in vitro and compared the efficiency and dynamics of transgene expression a ...
Leto: 2022 Vir: Fakulteta za elektrotehniko (UL FE)
Študij delovanja promotorjev genov PR1b in TGA2 iz krompirja
Tamara Polajžer, Kristina Gruden, Kristina Gruden, Darja Žgur-Bertok, Darja Žgur-Bertok, Matej Butala
Magistrsko delo
Oznake: PR1b;TGA2;krompir;transformacija;
Študij delovanja promotorjev genov PR1b in TGA2 iz krompirja
Leto: 2016 Vir: Digitalni repozitorij raziskovalnih organizacij Slovenije
Contactless electroporation induced by high intensity pulsed electromagnetic fields via distributed nanoelectrodes
Damijan Miklavčič, Vitalij Novickij, Matej Kranjc, Tamara Polajžer, Saša Haberl, Tina Batista Napotnik, Rok Romih, Darja Lisjak
Izvirni znanstveni članek
Oznake: magnetno polje;permeabilizacija membrane;zlati nanodelci;neinvazivna elektroporacija;nanodelci;magnetic fields;membrane permeabilization;gold nanoparticles;non-invasive electroporation;nanoparticles;
Pulsed electric fields (PEFs) can be used to transiently increase cell membrane permeability in procedures ranging from gene therapy to tumor eradication. Although very efficient, PEF-based therapies generally require the use of invasive electrodes, which cause pain and tissue damage. An emerging no ...
Leto: 2020 Vir: Fakulteta za elektrotehniko (UL FE)
Št. zadetkov: 7
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