Nacionalni portal odprte znanosti

Razvoj celičnih tovarn na osnovi bakterij iz rodu Streptomyces je ključen za proizvodnjo novih in že poznanih biološko aktivnih učinkovin. S postopno delecijo večjih delov genoma lahko razvijemo učinkovito platformo za heterologno izražanje sekundarnih metabolitov. Klasične metode za manipulacije ge ...

CRISPR-Cas9 technology has emerged as a promising tool for genetic engineering of Streptomyces strains. However, in practice, numerous technical hurdles have yet to be overcome when developing robust editing procedures. Here, we developed an extension of the CRISPR-Cas toolbox, a simple and reliable ...

Plant pan-genomes, which aggregate genomic sequences and annotations from multiple individuals of a species, have emerged as transformative tools for understanding genetic diversity, adaptation, and evolutionary dynamics. Super-pan-genomes, extending across species boundaries, further enable compara ...

Cultivar Désirée is an important model for potato functional genomics studies to assist breeding strategies. Here, we present a haplotype-resolved genome assembly of Désirée, achieved by assembling PacBio HiFi reads and Hi-C scaffolding, resulting in a high-contiguity chromosome-level assembly. We i ...