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Št. zadetkov: 8
Chloroplast redox state changes indicate cell-to-cell signalling during the hypersensitive response
Tjaša Lukan, Kristina Gruden, Anže Županič, Tjaša Mahkovec Povalej
Raziskovalni podatki
Oznake: krompir;krompirjev virus Y;stromule;analize slik;mikroskopija;celična smrt;prostorsko-časovne analize;cell signalling;chloroplasts;microscopy;ISA-tab;potato virus Y;redox responses;cell death;spatiotemporal analysis;
We performed detailed spatiotemporal analysis of chloroplast redox response to potato virus Y (PVY) infection in resistant Ny‐1-gene-bearing potato and its transgenic counterpart with impaired SA accumulation and compromised resistance. We found that the chloroplasts are highly oxidized in the cells ...
Leto: 2021 Vir: Nacionalni inštitut za biologijo (NIB)
Primerjava regeneracijske sposobnosti japonskega (Fallopia japonica) in češkega dresnika (Fallopia x bohemica)
Maša Krapež, Tjaša Mahkovec Povalej, Ana Miglioranza, Luen Zidar, Sonja Žabkar
Izvirni znanstveni članek
Oznake: regeneracijska sposobnost;potaknjenci;
Namen: Želeli smo primerjati regeneracijske sposobnosti potaknjencev japonskega dresnika (Fallopia japonica) in češkega dresnika (Fallopia x bohemica). Zanimal nas je tudi vpliv dolžine in starosti potaknjenega dela stebla na uspešnost regeneracije rastline. Metoda: V ločena korita, napolnjena z me ...
Leto: 2019 Vir: Biotehniška fakulteta (UL BF)
Chloroplast redox state changes mark cell-to-cell signaling in the hypersensitive response
Tjaša Lukan, Anže Županič, Tjaša Mahkovec Povalej, Jacob O. Brunkard, Mirjam Kmetič, Mojca Juteršek, Špela Baebler, Kristina Gruden
Izvirni znanstveni članek
Oznake: chloroplast redox state;hypersensitive response (HR)-conferred resistance;immune signaling;live cell imaging;Solanum tuberosum (potato);spatiotemporal analysis;stromules;virus resistance;
Hypersensitive response (HR)-conferred resistance is associated with induction of pro-grammed cell death and pathogen spread restriction in its proximity. The exact role of chloro-plastic reactive oxygen species and its link with salicylic acid (SA) signaling in HR remainunexplained. To unravel this ...
Leto: 2023 Vir: Nacionalni inštitut za biologijo (NIB)
Jasmonic acid and salicylic acid interact to determine spatial regulation of gene expression responses in potato leaf to herbivory by Colorado potato beetle and mechanical wounding
Valentina Levak, Tjaša Mahkovec Povalej, Karmen Pogačar, Katja Stare, Maja Zagorščak, Tim Hawkins, Joanne Robson, David Dobnik, Tjaša Lukan, Kristina Gruden
Izvirni znanstveni članek
Oznake: koloradski hrošč;jasmonska kislina;krompir;odziv rastlin na ranjenje;salicilna kislina;Colorado potato beetle;herbivore;hormonal crosstalk;jasmonic acid;potato;salicylic acid;wounding;
We investigated the spatial dynamics of potato (Solanum tuberosum) responses to herbivory and mechanical wounding. We first followed the spatiotemporal response of jasmonic acid (JA) signaling, known to be involved in the response. We generated two potato sensor lines: a JAZ degradation sensor and a ...
Leto: 2025 Vir: Nacionalni inštitut za biologijo (NIB)
Optimizacija priprave senzorskih rastlin krompirja za raziskave imunske signalizacije
Tjaša Mahkovec Povalej, Kristina Gruden, Kristina Gruden, Tjaša Lukan, Tjaša Lukan, Jasna Dolenc Koce, Jasna Dolenc Koce, Katarina Vogel-Mikuš
Magistrsko delo
Oznake: biološki senzor;citokinini;Jas9-VENUS;krompirjev virus Y;rastlinski hormoni;rastlinski imunski sistem;redoks stanje;roGFP;ROS;Solanum tuberosum;
Klasične biokemijske in vitro metode za proučevanja rastlinskih biotskih interakcij so zamudne, invazivne in omogočajo vpogled le v posamezno stanje celice. Zato je ključen razvoj biosenzorjev, ki omogočajo neinvazivno spremljanje rastlinskega imunskega odziva in vivo z visoko časovno in prostorsko ...
Leto: 2020 Vir: Digitalni repozitorij raziskovalnih organizacij Slovenije
CRISPR/Cas9-mediated fine-tuning of miRNA expression in tetraploid potato
Tjaša Lukan, Florian Veillet, Maja Križnik, Anna Coll Rius, Tjaša Mahkovec Povalej, Karmen Pogačar, Katja Stare, Laura Chauvin, Jean-Eric Chauvin, Kristina Gruden
Izvirni znanstveni članek
Oznake: CRISPR/Cas9;hypersensitive response (HR)-conferred resistance;immune signaling;live cell imaging;Solanum tuberosum (potato);spatiotemporal analysis;stromules;virus resistance;
MicroRNAs (miRNAs) are small noncoding RNAs, which modulate the abundance and spatiotemporal accumulation of target mRNAs at transcriptional and post-transcriptional levels and through that play important roles in several biological processes in plants. Here we show that in polyploid species, CRISPR ...
Leto: 2022 Vir: Nacionalni inštitut za biologijo (NIB)
ǂThe ǂStPti5 ethylene response factor acts as a susceptibility factorby negatively regulating the potato immune responseto pathogens
Anna Coll Rius, Tjaša Lukan, Katja Stare, Maja Zagorščak, Tjaša Mahkovec Povalej, Špela Baebler, Salomé Prat, Núria Sánchez Coll, Marc Valls, Marko Petek, Kristina Gruden
Izvirni znanstveni članek
Oznake: ethylene response factor;immune signalling;potato virus Y;Pti5;Ralstonia solanacearum;Solanum tuberosum;susceptibility factor;
Ethylene response factors (ERFs) have been associated with biotic stress in Arabidopsis, while their function in non-model plants is still poorly understood. Here we investigated the role of potato ERF StPti5 in plant immunity. We show that StPti5 acts as a susceptibility factor. It negatively regul ...
Leto: 2024 Vir: Nacionalni inštitut za biologijo (NIB)
Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal region
Anže Vozelj, Tjaša Mahkovec Povalej, Katja Stare, Magda Tušek-Žnidarič, Katarina Bačnik, Valentina Levak, Ion Gutierrez-Aguirre, Marjetka Podobnik, Kristina Gruden, Anna Coll Rius, Tjaša Lukan
Izvirni znanstveni članek
Oznake: krompirjev virus Y;krompir;plaščni proteini;gibanje virusa;točkovne mutacije;potato virus Y;potato;coat proteins;viral movement;point mutations;
Potato virus Y (PVY) is one of the top 10 economically most important plant viruses and responsible for major yield losses. We previously suggested the involvement of the N-terminal region of PVY coat protein (CP) in PVY spread. By constructing different N-terminal deletion mutants of the PVY N605 s ...
Leto: 2025 Vir: Nacionalni inštitut za biologijo (NIB)
Št. zadetkov: 8
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