Št. zadetkov: 10
Izvirni znanstveni članek
Oznake:
test performance study;Phyllosticta citricarpa;real time PCR;TEF1;biotechnology;
Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, significantly affects citrus fruit marketability and can lead to premature fruit drop. Accurate and reliable detection of this quarantine pathogen is crucial, particularly for asymptomatic plant material. This study evaluated two ...
Leto:
2025
Vir:
Nacionalni inštitut za biologijo (NIB)
Končno poročilo o rezultatih raziskav
Oznake:
diagnostika;validacija metod;begomoviruses;EURL-Virology;
Detection of begomoviruses capable of infecting tomatoes and plants of the family Cucurbitaceae.
Leto:
2023
Vir:
Nacionalni inštitut za biologijo (NIB)
Kratki znanstveni prispevek
Oznake:
detection;identification;plant virus disease;
In July 2021, during an official survey for Tomato brown rugose fruit virus (ToBRFV), a sample composed of leaves and fruit was taken from three tomato (Solanum lycopersicum cv. Factor F1) plants growing in a greenhouse producing fresh tomatoes in central Slovenia. The sampled plants were slightly d ...
Leto:
2022
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
real-time LAMP;grapevine yellows phytoplasma;validation;
A fluorescence-based real-time loop-mediated isothermal amplification (LAMP) assay for ‘Candidatus Phytoplasama solani’ (Bois noir phytoplasma; BNp) detection was developed and optimised for rapid laboratory and on-site BNp detection. This assay is highly specific, rapid and as sensitive as qPCR. It ...
Leto:
2017
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
PSTVd;PepMV;seeds;water;concentration;loop-mediated isothermal amplification;
Waterborne and seedborne Pepino mosaic virus (PepMV) and Potato spindle tuber viroid (PSTVd) pose serious threats to tomato production due to seed transmission and mechanical transmission, coupled with their long-term stability outside the host plant. Therefore, rapid and sensitive diagnostic proced ...
Leto:
2017
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
fitoplazme;leska;trta;zlata trsna rumenica;bolezni vinske trte;rastlinske bolezni;phytoplasmas;haselnuts;grapevine;Flavescence dorée;epidemiology;plant disease;
Flavescence dorée (FD) phytoplasma from 16SrV-C and -D subgroups cause severe damage to grapevines throughout Europe. This phytoplasma is transmitted from grapevine to grapevine by the sap-sucking leafhopper Scaphoideus titanus. European black alder and clematis serve as perennial plant reservoirs f ...
Leto:
2023
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
absolute quantification;AAV;gene therapy;electron microscopy;digital PCR;real-time PCR;
One of the main challenges in the gene therapy viral vector development is to establish an optimized process for its large scale production. This requires optimization for upstream and downstream processes as well as methods that enable the step-by step analytical characterization of the virus, the ...
Leto:
2019
Vir:
Nacionalni inštitut za biologijo (NIB)
Mojca Janc,
Kaja Zevnik,
Ana Dolinar,
Tjaša Jakomin,
Maja Štalekar,
Katarina Bačnik,
Denis Kutnjak,
Magda Tušek-Žnidarič,
L. Zentilin,
Dmitri G. Fedorov,
David Dobnik
Izvirni znanstveni članek
Oznake:
rekombinantni z adenovirusi povezani virusi;centrifugiranje v CsCl gradientu;analitične metode;digitalni kapljični PCR;virologija;recombinant adeno-associated viruses (rAAVs);CsCl ultracentrifugation gradient;analytical methods;digital droplet PCR (ddPCR);transmission electron microscopy (TEM);analytical ultracentrifugation (AUC);size-exclusion chromatography coupled with multi-angle light scattering (SEC-MALS);Illumina sequencing;virology;
Recombinant adeno-associated viruses (rAAVs) play a pivotal role in the treatment of genetic diseases. However, current production and purification processes yield AAV-based preparations that often contain unwanted empty, partially filled or damaged viral particles and impurities, including residual ...
Leto:
2024
Vir:
Nacionalni inštitut za biologijo (NIB)
Špela Alič,
Géraldine Anthoine,
A Chabirand,
Anne-Marie Chappé,
Tanja Dreo,
Tjaša Jakomin,
L Laurenson,
Tadeja Lukežič,
Nataša Mehle,
E Metz-Verschure,
H Mouaziz,
J Oorspronk,
Manca Pirc,
Maja Ravnikar,
Naomi te Braak,
J. Tomlinson,
Marcel Westenberg
Elaborat, predštudija, študija
Oznake:
pests;tests;
We prepared a list of methods and tests for validation in test performance study (TPS) Round 1, both for laboratory and on-site use, for 6 selected pests: Erwinia amylovora, Pantoea stewartiisubsp. stewartii, citrus tristeza virus, plum pox virus, Fusarium circinatum and Bursaphelenchus xylophilus. ...
Leto:
2019
Vir:
Nacionalni inštitut za biologijo (NIB)
Nataša Mehle,
Manca Pirc,
Ana Vučurović,
Marjana Camloh,
Denis Kutnjak,
Anja Pecman,
Špela Alič,
Aleksander Benčič,
Vladimir Grujić,
Neža Turnšek,
Janja Matičič,
Špela Prijatelj-Novak,
Aleš Blatnik,
Jakob Brodarič,
Irena Bajde,
Veronika Bukvič,
Tjaša Jakomin,
Tanja Dreo
Končno poročilo o rezultatih raziskav
Oznake:
diagnostika;virusi;bakterije;fitoplazme;varstvo rastlin;
Leto:
2025
Vir:
Nacionalni inštitut za biologijo (NIB)