Št. zadetkov: 9
Magistrsko delo
Oznake:
Keratin 5;keratinociti;koža;bulozna epidermoliza simpleks;togost celične membrane;agregati;tkivni model kože;
Keratini (K) so največja skupina intermediarnih filamentov. Zaradi svoje strukture se organizirajo v navite dimere. K5 tvori heterodimerne strukture s K14 in v veliki meri se izražata v bazalnih keratinocitih v epidermisu kože. Njuna glavna vloga je fizična stabilnost epidermisa. Določene mutacije v ...
Leto:
2024
Vir:
Biotehniška fakulteta (UL BF)
Magistrsko delo
Oznake:
adhesom;kolagen VII;dominantna distrofična bulozna epidermoliza;recesivna distrofična bulozna epidermoliza;inducirane pluripotentne matične celice;citoskelet;zunajcelični matriks;masna spektrometrija;
Kolagen VII je protein zunajceličnega matriksa (ECM), kodiran z genom COL7A1. Tvori sidrne fibrile, ki se raztezajo od dna bazalne lamine do papilarnega dermisa, in zagotavljajo integriteto dermalno-epidermalnega stika. Mutacije v COL7A1 vodijo do pojava distrofične bulozne epidermolize (DEB). Gre z ...
Leto:
2024
Vir:
Biotehniška fakulteta (UL BF)
Magistrsko delo
Oznake:
keratin 14;keratinociti, koža;bulozna epidermoliza simpleks;adhezija keratinocitov;proteini adhezoma;tkivni model kože;
Keratini (K) so proteini intermediarnih filamentov, ki v keratinocitih oblikujejo keratinski citoskelet, ki zagotavlja mehansko stabilnost epidermisa kože. V bazalnih celicah epidermisa se izražata K14 in K5, ki skupaj tvorita heterodimere. Mutacije v genu za K14 so povezane z nastankom bulozne epid ...
Leto:
2026
Vir:
Medicinska fakulteta (UL MF)
Doktorska disertacija
Oznake:
keratinski citoskelet;celični odziv;ionizirajoče sevanje;Citologija;Disertacije;Deformiran citoskelet;Epitelne celice;Keranocite;Kožne bolezni;Keracinom bazalnih celic;Izpostavljenost sončnemu sevanju;Ionizirajoče sevanje;Citokini receptorjev smrti;Molekularna genetika;Mutacije keratina;Izražanje genov;
Keratini so proteini intermediarnih filamentov, ki so izraženi v vseh tipih epitelijskih celic. Keratinske mutacije povzročajo številne hude dedne bolezni krhkosti kože. Med njimi je tudi bulozna epidermoliza simpleks (EBS), povezana z mutacijami v keratinih 5 in 14. Mutirani keratini tvorijo deform ...
Leto:
2020
Vir:
Medicinska fakulteta (UL MF)
Izvirni znanstveni članek
Oznake:
CRISPR-Cas9;epidermolysis bullosa simplex;homology-directed repair (HDR);induced pluripotent stem cells (iPSC);isogenic control;KRTS;
Severe epidermolysis bullosa simplex is a skin fragility disorder characterized by blistering caused by cytolysis within basal keratinocytes, resulting in compromised epidermal integrity. Here we report the generation of the human induced pluripotent stem cell (hiPSC) line MLi002-A-1, an isogenic co ...
Leto:
2026
Vir:
Medicinska fakulteta (UL MF)
Izvirni znanstveni članek
Oznake:
glioblastom;23/5000 matične celice glioblastoma;biomarkerji;glioblastoma;glioblastoma stem cells;biomarkers;
Glioblastoma (GB) is the most aggressive brain malignancy. Although some potential glioblastoma biomarkers have already been identified, there is a lack of cell membrane-bound biomarkers capable of distinguishing brain tissue from glioblastoma and/or glioblastoma stem cells (GSC), which are responsi ...
Leto:
2018
Vir:
Medicinska fakulteta (UL MF)
Izvirni znanstveni članek
Oznake:
matične celice;epidermoliza;kolagen VII;stem cell;epidermolysis;collagen VII;
We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier ...
Leto:
2021
Vir:
Medicinska fakulteta (UL MF)
Izvirni znanstveni članek
Oznake:
keratin;epidermoliza bullosa simplex;mutacija;epidermolysis bullosa simplex;mutation;
Keratins are one of the most abundant proteins in epithelial cells. They form a cytoskeletal filament network whose structural organization seriously conditions its function. Dynamic keratin particles and aggregates are often observed at the periphery of mutant keratinocytes related to the hereditar ...
Leto:
2020
Vir:
Medicinska fakulteta (UL MF)
Izvirni znanstveni članek
Oznake:
human pluripotent stem cells;suspension culture;aggregate uniformity;HA-100;manufacturing;TJP1;
Human pluripotent stem cell (hPSC) manufacturing workflows frequently rely on suspension aggregation, yet inter-line and batch-to-batch variability in aggregate formation can compromise process consistency and downstream differentiation performance. We evaluated whether a short exposure to HA-100, a ...
Leto:
2026
Vir:
Medicinska fakulteta (UL MF)