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Št. zadetkov: 3
ǂThe ǂdigital MIQE guidelines update
Jim F. Huggett, Alexandra S. Whale, Ward De Spiegelaere, Afif M. Abdel Nour, Young-Kyung Bae, Vladimír Beneš, Dan Burke, Megan Cleveland, Philippe Corbisier, Alison S. Devonshire, Lianhua Dong, Daniela Drandi, Carole A. Foy, Jeremy A. Garson, Hua-Jun He, Jan Hellemans, Mikael Kubista, Antoon Lievens, Mike G. Makrigiorgos, Mojca Milavec, Reinhold D. Mueller, Tania Nolan, Denise M. O'Sullivan, Michael W. Pfaffl, Stefan Rödiger, Erica L. Romsos, Gregory L. Shipley, Valérie Taly, Andreas Untergasser, Carl T. Wittwer, Stephen A. Bustin, Jo Vandesompele
Pregledni znanstveni članek
Oznake: biokemija;digitalna PCR;dPCR;MIQE;dMIQE;
Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments ...
Leto: 2020 Vir: Nacionalni inštitut za biologijo (NIB)
Digital PCR for the characterization of reference materials
Megan H. Cleveland, Hua-Jun He, Mojca Milavec, Young-Kyung Bae, Peter M. Vallone, Jim F. Huggett
Pregledni znanstveni članek
Oznake: digitalna PCR;digitalna verižna reakcija s polimerazo;referenčni materiali;UV-spektroskopija;digital PCR;dPCR;reference materials;UV-spectroscopy;
Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characteri ...
Leto: 2024 Vir: Nacionalni inštitut za biologijo (NIB)
ǂThe ǂapplication of digital PCR as a reference measurement procedure to support the accuracy of quality assurance for infectious disease molecular diagnostic testing
Samreen Falak, Denise M O’Sullivan, Megan H. Cleveland, Simon Cowen, Eloise J. Busby, Alison S. Devonshire, Esmeralda Valiente, Gerwyn M. Jones, Martin Kammel, Mojca Milavec
Izvirni znanstveni članek
Oznake: nucleic acid amplification tests;dPCR;reference measurement procedure;external quality assessment;SARS-CoV-2;
BACKGROUND: Nucleic acid amplification tests (NAATs) assist in the diagnosis of numerous infectious diseases. They are typically sensitive and specific and can be quickly developed and adapted. Far more challenging is the development of standards to ensure NAATs are performing within specification; ...
Leto: 2025 Vir: Nacionalni inštitut za biologijo (NIB)
Št. zadetkov: 3
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