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Št. zadetkov: 20
Novel approaches for detection and quantification of genetically modified organisms (GMOs)
Alexandra Bogožalec Košir
Video in druga učna gradiva
Oznake: science;scientific research;biology
Leto: 2017 Vir: videolectures.net
Priprava, vrednotenje in uporaba kontrolnih materialov za določanje koncentracije virusa opičjih koz z verižno reakcijo s polimerazo
Mija Šulin, Alexandra Bogožalec Košir
Magistrsko delo
Oznake: dPCR;kontrolni material;virus opičjih koz;
Za zanesljivo diagnostiko z molekularnimi metodami potrebujemo kontrolne materiale, ki med drugim omogočajo primerjavo rezultatov med laboratoriji. Uporaba kontrolnih materialov je zelo pomembna pri razvoju novih testov, natančni kvantifikaciji genetskega materiala, z njimi tudi upoštevamo in kompen ...
Leto: 2024 Vir: Fakulteta za kemijo in kemijsko tehnologijo (UL FKKT)
Digital PCR-based genotyping: a precision approach to HCMV drug resistance
Mojca Milavec, Tašja Cvelbar, Alexandra Bogožalec Košir
Samostojni znanstveni sestavek ali poglavje v monografski publikaciji
Oznake: digital PCR;human cytomegalovirus;antimicrobial drug resistance;mutations;
The genotyping workflow described uses digital PCR (dPCR) to detect and quantify drug resistance mutations in human cytomegalovirus (HCMV). The method focuses on the detection and quantification of three common mutations in the UL97 gene at codons 460, 594, and 595, which are responsible for the maj ...
Leto: 2025 Vir: Nacionalni inštitut za biologijo (NIB)
Digital PCR genotyping of pepino mosaic virus
Amadej Jelenčič, David Dobnik, Alexandra Bogožalec Košir, Nataša Mehle
Samostojni znanstveni sestavek ali poglavje v monografski publikaciji
Oznake: plant viruses;detection;quantification;Pepino mosaic virus;PepMV;digital PCR;dPCR;RT-dPCR;duplex;
Pepino mosaic virus (PepMV) is a plant pathogen causing significant economic losses in tomato production. Sensitive, reliable, and robust detection methods are crucial for containing the spread of PepMV and reducing its damaging effects. Digital PCR (dPCR) presents several advantages to conventional ...
Leto: 2025 Vir: Nacionalni inštitut za biologijo (NIB)
Evaluation of DNA extraction methods for reliable quantification of Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa
Alexandra Bogožalec Košir, Dane Lužnik, Viktorija Tomič, Mojca Milavec
Izvirni znanstveni članek
Oznake: nukleinske kisline;dPCR;metode ekstrakcije DNK;gram-negativne bakterije;nucleic acid;DNA extraction methods;Gram-negative bacteria;
Detection and quantification of DNA biomarkers relies heavily on the yield and quality of DNA obtained by extraction from different matrices. Although a large number of studies have compared the yields of different extraction methods, the repeatability and intermediate precision of these methods hav ...
Leto: 2023 Vir: Nacionalni inštitut za biologijo (NIB)
One-step reverse-transcription digital PCR for reliable quantification of different pepino mosaic virus genotypes
Nataša Mehle, Larisa Gregur, Alexandra Bogožalec Košir, David Dobnik
Izvirni znanstveni članek
Oznake: digital PCR;pepino mosaic virus;quantification;genotype specific;
In recent years, pepino mosaic virus (PepMV) has rapidly evolved from an emerging virus to an endemic pathogen, as it causes significant loses to tomato crops worldwide. At present, the main control strategy for prevention of PepMV disease in tomato production remains based on strict hygiene measure ...
Leto: 2020 Vir: Nacionalni inštitut za biologijo (NIB)
Digital PCR as an effective tool for GMO quantification in complex matrices
Alexandra Bogožalec Košir, Tina Demšar, Dejan Štebih, Jana Žel, Mojca Milavec
Izvirni znanstveni članek
Oznake: genetically modified organisms;digital PCR;GMO quantification;complex matrices;
The increased use of genetically modified organisms (GMOs) is accompanied by increased complexity of the matrices that contain GMOs. The most common DNA-based approach for GMO detection and quantification is real-time quantitative polymerase chain reaction (qPCR). However, as qPCR is sensitive to in ...
Leto: 2019 Vir: Nacionalni inštitut za biologijo (NIB)
Integrative high-throughput sequencing techniques for microbial genomics
Aleksander Benčič, Tanja Dreo, Alexandra Bogožalec Košir, Mojca Milavec, Maja Zagorščak
Doktorska disertacija
Oznake: visokozmogljivo sekvenciranje;gemonski pristopi;mikrobiom;doktorske disertacije;high-throughput sequencing techniques;microbiome;
Leto: 2025 Vir: Nacionalni inštitut za biologijo (NIB)
Vrednotenje različnih metod ekstrakcije nukleinskih kislin iz humane celične 3D kulture raka
Stefanija Ivanova, Alexandra Bogožalec Košir, Metka Novak, Barbara Breznik, Mojca Milavec
Objavljeni znanstveni prispevek na konferenci
Oznake: celične kulture;tumorji;molekularna diagnostika;ekstrakcijske metode;kontrola kakovosti;GenomeMET;
Leto: 2025 Vir: Nacionalni inštitut za biologijo (NIB)
Development and inter-laboratory assessment of droplet digital PCR assays for multiplex quantification of 15 genetically modified soybean lines
Alexandra Bogožalec Košir, Bjørn Spilsberg, Arne Holst-Jensen, Jana Žel, David Dobnik
Izvirni znanstveni članek
Oznake: droplet digital PCR;genetically modified organisms;soybean;
Quantification of genetically modified organisms (GMOs) in food and feed products is often required for their labelling or for tolerance thresholds. Standard-curve-based simplex quantitative polymerase chain reaction (qPCR) is the prevailing technology, which is often combined with screening analysi ...
Leto: 2017 Vir: Nacionalni inštitut za biologijo (NIB)
Št. zadetkov: 20
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