Št. zadetkov: 16
Video in druga učna gradiva
Oznake:
science;scientific research;biology
Leto:
2017
Vir:
videolectures.net
Magistrsko delo
Oznake:
dPCR;kontrolni material;virus opičjih koz;
Za zanesljivo diagnostiko z molekularnimi metodami potrebujemo kontrolne materiale, ki med drugim omogočajo primerjavo rezultatov med laboratoriji. Uporaba kontrolnih materialov je zelo pomembna pri razvoju novih testov, natančni kvantifikaciji genetskega materiala, z njimi tudi upoštevamo in kompen ...
Leto:
2024
Vir:
Fakulteta za kemijo in kemijsko tehnologijo (UL FKKT)
Samostojni znanstveni sestavek ali poglavje v monografski publikaciji
Oznake:
digital PCR;human cytomegalovirus;antimicrobial drug resistance;mutations;
The genotyping workflow described uses digital PCR (dPCR) to detect and quantify drug resistance mutations in human cytomegalovirus (HCMV). The method focuses on the detection and quantification of three common mutations in the UL97 gene at codons 460, 594, and 595, which are responsible for the maj ...
Leto:
2025
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
nukleinske kisline;dPCR;metode ekstrakcije DNK;gram-negativne bakterije;nucleic acid;DNA extraction methods;Gram-negative bacteria;
Detection and quantification of DNA biomarkers relies heavily on the yield and quality of DNA obtained by extraction from different matrices. Although a large number of studies have compared the yields of different extraction methods, the repeatability and intermediate precision of these methods hav ...
Leto:
2023
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
digital PCR;pepino mosaic virus;quantification;genotype specific;
In recent years, pepino mosaic virus (PepMV) has rapidly evolved from an emerging virus to an endemic pathogen, as it causes significant loses to tomato crops worldwide. At present, the main control strategy for prevention of PepMV disease in tomato production remains based on strict hygiene measure ...
Leto:
2020
Vir:
Nacionalni inštitut za biologijo (NIB)
Samostojni znanstveni sestavek ali poglavje v monografski publikaciji
Oznake:
plant viruses;detection;quantification;Pepino mosaic virus;PepMV;digital PCR;dPCR;RT-dPCR;duplex;
Pepino mosaic virus (PepMV) is a plant pathogen causing significant economic losses in tomato production. Sensitive, reliable, and robust detection methods are crucial for containing the spread of PepMV and reducing its damaging effects. Digital PCR (dPCR) presents several advantages to conventional ...
Leto:
2025
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
genetically modified organisms;digital PCR;GMO quantification;complex matrices;
The increased use of genetically modified organisms (GMOs) is accompanied by increased complexity of the matrices that contain GMOs. The most common DNA-based approach for GMO detection and quantification is real-time quantitative polymerase chain reaction (qPCR). However, as qPCR is sensitive to in ...
Leto:
2019
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
droplet digital PCR;genetically modified organisms;soybean;
Quantification of genetically modified organisms (GMOs) in food and feed products is often required for their labelling or for tolerance thresholds. Standard-curve-based simplex quantitative polymerase chain reaction (qPCR) is the prevailing technology, which is often combined with screening analysi ...
Leto:
2017
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
digitalna PCR;Gram-negativne bakterije;detekcija patogenov;okužba dihal;biomarkerji;diagnostika;molekularna biologija;digital PCR (dPCR);Gram-negative bacteria;pathogen detection;respiratory infections;biomarkers;RUCS algorithm;
Rapid and accurate identification of bacterial pathogens is crucial for effective treatment and infection control, particularly in hospital settings. Conventional methods like culture techniques and MALDI-TOF mass spectrometry are often time-consuming and less sensitive. This study addresses the nee ...
Leto:
2024
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
Xylophilus ampelinus;grapevine bacterial blight;molecular diagnostics;Vitis vinifera;real-time PCR;genome-informed assay development;
Background Xylophilus ampelinus is a plant pathogenic bacterium that causes bacterial blight in grapevines, which can lead to severe yield losses and economic damage. Owing to its fastidious growth on culture media, detection is primarily based on molecular methods. However, existing tests have prod ...
Leto:
2025
Vir:
Nacionalni inštitut za biologijo (NIB)